Reconstitution Values
Enter your peptide vial and water amounts
Total mg in your vial
Amount you want to inject per dose
(BAC Water, Sterile Water, Acetic Acid)
Determines peptide concentration
Your Results
Dosing Summary
Syringe Comparison
See how your 0 units looks on each syringe size
✓ Recommended for this dose
Schedule & Supply
0
Doses Per Vial
0.0
Weeks Per Vial
5
Doses Per Week
0
mcg/week
Step-by-Step: How to Reconstitute Peptides
Follow these steps carefully to properly reconstitute lyophilized (freeze-dried) research peptides.
Gather Your Supplies
You will need: a vial of lyophilized peptide, bacteriostatic water (BAC water), alcohol wipes, a 3mL Luer lock syringe for reconstitution, and an insulin syringe for dosing. Have a clean, well-lit workspace ready.
Calculate Your Volume
Determine how much bacteriostatic water to add based on your desired concentration. For example, adding 2mL of BAC water to a 10mg peptide vial gives you 5mg/mL (5,000 mcg/mL). Use the calculator above to find your ideal ratio.
Prepare the Vial
Remove the plastic cap from the peptide vial. Wipe the rubber stopper with an alcohol swab and let it air dry for 10–15 seconds. Do not blow on it or touch the stopper after cleaning.
Draw the Bacteriostatic Water
Using a 3mL syringe, draw the calculated amount of BAC water. Pull back the plunger to the correct marking. Remove any air bubbles by gently tapping the syringe and pushing them out.
Inject Water Into the Vial
Insert the needle through the rubber stopper at a slight angle. Slowly release the water down the inside wall of the vial — do NOT spray it directly onto the peptide powder, as this can damage the compound. Let the water trickle gently.
Mix Gently
Once the water is added, gently swirl the vial in a circular motion. Never shake the vial, as this can denature the peptide. The powder should dissolve within 1–3 minutes. If small particles remain, let it sit in the refrigerator for 15–30 minutes.
Store Properly
Store the reconstituted peptide in a refrigerator at 2–8°C. Keep the vial upright and away from direct light. Reconstituted peptides are generally stable for up to 28 days, though many researchers report stability for much longer with proper storage. Label the vial with the date and concentration.
Common Mistakes to Avoid
- Spraying water directly onto the peptide powder — always let it run down the vial wall
- Shaking the vial vigorously instead of gently swirling
- Using regular tap or distilled water instead of bacteriostatic water
- Touching the rubber stopper or needle tip with your fingers
- Storing reconstituted peptides at room temperature or in direct sunlight
- Reusing syringes or needles across different vials
Reconstitution FAQ
Answers to the most common questions about peptide reconstitution.